Nucleotide sequence of three tRNA genes from tomato chloroplast genome.

Clones including tRNA genes from tomato {Lycopersicon esculentum) chloroplast DNA have been obtained. Here we report the nucleotide sequence of tmH\ trnD-^ and tmL\ Sequences were determined by the dideoxy method (1). In higher plant chloroplast genomes, tmY and trnD are clustered together with trnE in the large single copy (LSQ region (2). The sequence of trnY reveals 100% identity with its counterparts in pea (3), spinach (4) and tobacco (5), and it is highly similar with trnY of rice (6), wheat (7), the beechdrop Epiphagus virginiana (8), and the liverwort Marchantia polymorpha (9). Aside from tobacco (5), tomato trnD is identical to the corresponding genes in the monocots rice and wheat (6, 10), while it slightly differs from the dicots spinach (11), pea (12) and Epiphagus (8), and the liverwort Marchantia (9). The trnL gene is located in the small single copy (SSC) region between rpl32 and ORF313. It has 100% homology to trnL of tobacco and also high similarity to its counterparts in rice (6), Epiphagus (13), liverwort (9) and the corresponding gene product from spinach (14). ACKNOWLEDGEMENTS